![]() So only when you have no flexibility in your choice of primers, I would use SnapGene to design them. Go to the Primer design in SnapGene tutorial to see how you can use SnapGene to design primers Primer specificity: the ability of a primer to bind to a single or to multiple regions in the genome It does not take into account the ability of primers to form hairpins or dimers nor does it check the specificity of the primers. SnapGene is not the ideal tool for designing primers. Go to the Changing what you see in SnapGene tutorial to see how you can switch between views, change a view, show additional info. See the introduction tutorial video and the working with features tutorial video for further details on how to annotate sequences in SnapGene.
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